Ten-man New Edubiase United exited the CAF Confederation Cup after losing 5-4 on penalties to Congolese side Diables Noirs on Saturday in Brazzaville.
The 2012 MTN Ghana FA Cup winners lost 1-0 at the death of the match after Mahmoud Suleman was sent off in the second half.
Both sides were tied 1-1 on aggregate.
During the penalty shoot-outs, Two players-Abdul Rahman and striker Adams Basit-missed their kicks but goalkeeper Eric Nii Baah made a save.
Edubiase were making their debut in Africa but could not progress from the preliminary qualifying round.